What version of Handbrake? What OS?

Might try removing, redownloading, and re-importing the Preset.

I've used the Preset before on Linux Handbrake with good success. Unfortunately, some of my 720p Blu Ray rips don't work. Video is fine but audio sounds like it has been run through a slow motion machine and all the voices are extremely deep and explosions sound like Ace Ventura.

I'll be posting a script up later that I've gotten some very good quality x264 encoding with.. but it'll only work on Linux I believe.