When I was interviewing with Google for a Sr. Systems Engineer position, one of the first questions they asked was "Know Ruby?" followed by "Know Ruby on Rails?" I guess Google is using it enough to use it for screening candidates.

Python is being used in place of Perl on servers by T-Mobile and other telecoms. When you IM or SMS from your device, we were using Python to check the device timestamp, server timestamp, and sequence IDs to keep conversations in check. This makes alot more sense when you fire off 5 IMs in a row, the server received 2 but queued 3 when your phone reacquired a connection.